マウス・ラット用
無加温型非観血式血圧計
Blood Pressure Monitor for Mice and Rats
Capable of recording from neonatal mice (1.4 g) to the aged rats (1 kg)
ラットやマウス等の小動物の血圧を測定する場合、従来の非観血式血圧計では何らかの方法により動物を暖めておく必要がありました。これに対しMK-2000STは室温が23℃以上あれば自然の(無加温の）状態のままで測定を行なうことができます。薬物の影響を調べるのに最適な装置であり、尾動脈圧の経時変化をモニタすることもできるなど従来の非観血式血圧計の概念を覆す画期的な装置です。操作は至って簡単で、動物を測定台にセットした後はボタンを押すだけの全自動測定が行なえます。
主な特長
光電脈波法により検出された尾動脈の脈波の振幅をコンピュータがとらえ、そのピークをディスプレイ上にプロットしていきます。縦軸に脈波の振幅、横軸にカフ圧が表示されます。この方式を採用することにより、画面上でノイズと信号の識別が容易となり、より信頼性の高い血圧測定を行なうことができます。
２種類のモードを選択して測定できます。
| MODE1 | HR | SBP | ||
| MODE2 | HR | SBP | MBP | (DBP) |
注：HR(脈拍数)、SBP(収縮期血圧)、MBP(平均血圧)、DBP(拡張期血圧、計算値)
スタートスイッチを押すと大まかなSBPを求めて約30mmHg/secで加圧していきます。その後数mmHg/secで減圧してSBPを求めます。MODE2では更にカフ圧を間欠的に降下させ、脈波の最大振幅点(MBP)を求めます。DBPはSBPおよびMBPから求めた計算値です。
計算式：(3MBP-SBP)÷2
連続測定機能･････最高10回まで連続して自動測定を行なうことができます。
高速印字機能･････高速で音の静かなサーマルプリンタを採用しました。平均値(AV)やSD値を含めた全てのデータが約1秒間で印字されます。又、実験者の判断により測定データの削除を行なうこともできます。
これまで測定が困難であったC57BL6のような有色マウスやネオネイタル・マウス(1.4g)、ハムスター（麻酔下）及びモルモット（覚醒時）、マーモセット等でも測定できます。
ショック状態の動物でも測定が可能です。
マルチチャンネルはもう不要です。アニマルホルダを複数用意しておけば次から次と測定できます。
モニタ機能･････設定された測定間隔(1〜99min)と測定回数に応じて、一匹の実験動物の尾動脈圧を経時的に測定し、データの印字及びパソコンへの転送までの一連の作業を全自動で行う機能が備わっています。
タイムスタンプ機能･････データ印字の際に計測時刻も印字されます。
画面コピー機能･････PrtScreenキーを使用することにより画面のハードコピーを行うことができます。
RS-232Cインターフェースが標準装備されており、パーソナルコンピュータにデータを転送することができます。データ受信プログラム(DCS-2000 Windows版)をオプションとして用意しました。これを使用することにより、得られたデータをエクセル等の表計算ソフトでＣＳＶファイルとして保存することができます。
Correlation between simultaneously recorded blood pressures (BP) from carotid catheter and BP measured with the Model MK-2000 in mice. Good correlation was seen in a wide range of BP, which was induced by i.v. infusions of pressor or depressor agents.
The data was offered courtesy of
Prof. Tomoyuki Kuwaki, Ph.D.,
Department of Molecular and Integrative
Physiology, Graduate School of Medicine,
Chiba University
| 本 体 | |
| SBP | パルス出現点のカフ圧 |
| MBP | 減圧時におけるパルスの最大振幅点のカフ圧 |
| DBP | 計算値 (3MBP-SBP)/2 |
| センサ部 | LED, フォトトランジスタ |
| 加圧範囲 | 0 - 350mm Hg |
| 記 録 紙 | 感熱記録紙、58mm幅(TSP-10) |
| 印字内容 | 日付、時間、動物番号、体重 測定モード、測定間隔、測定回数 測定項目及び平均値とSD値、計測時刻 |
| 電 源 | AC100V 50/60Hz |
| 外形寸法 | 210W × 310D × 210 mmH |
| 重 量 | 約 8 kg |
| キーボード | |
| キー | 33 |
| 外形寸法 | 145W × 120D × 20 mmH |
| 重 量 | 約 0.4 kg |
| 標 準 構 成 | |
| 本体(プリンタ含む) | 1 |
| キーボード | 1 |
| ラット用測定台 | 1 |
| アニマルホルダ(任意の1ｹ選択) | 1 |
| カフパルス・センサ(任意の1ｹ選択) | 1 |
| TSP-10 記録紙 （5巻入） | 1 |
| APC-2000 アクセサリケース | 1 |
| ダストカバー | 1 |
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＊ 特別仕様のアニマルホルダも製作致します。
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外観及び仕様は改良のため予告なしに変更する場合がありますのでご了承願います。
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Animal used : ddY Mouse
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Takahiro Iwamoto1,3,
Satomi Kita1,5,
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Mordecai P Blaustein2,
Yuji Arai4,
Shigeru Yoshida5,
Koji wakimoto6,
Issei Komuro7&Takeshi
Katsuragi1
1 Department of Pharmacology, School of Medicine, Fukuoka University, Fukuoka
814-0180, Japan.
2 Department of Physiology, University of Maryland School of Medicine,
Baltimore, Maryland 21201, USA.
3 Department of Molecular Physiology, National Cardiovascular Center Research
Institute, Osaka 565-8565, Japan.
4 Department of Bioscience, National Cardiovascular Center Research Institute,
Osaka 565-8565, Japan.
5 Medicinal Research Laboratories, Taisho Pharmaceutical Co., Ltd., Saitama
330-8530, Japan.
6 Discovery Research Laboratory, Tanabe Seiyaku Co., Ltd., Osaka 532-8505,
Japan.
7 Department of Cardiovascular Science and Medicine, Chiba University Graduate
School of Medicine, Chiba 260-8670, Japan.
Animal used : C57BL6 Mouse
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Jingui Yu, MD*, Koji Ogawa, MD, Yasuyuki Tokinaga, MD*, Shizue Iwahashi, MD*
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Kimiyasu Egami, MD; Satoshi Shintani, MD; Yosuke Katsuda, MD; Hisao Ikeda, MD;
Yo-ichi Nabeshima, MD; Tsutomu Imaizumi, MD
From the Cardiovascular Research Institute (T.S., Y.T., T.M.,
K.-i.S., K.E., S.S., Y.K., H.I., T.I.), Kurume University, Kurume; the
Department of Cardiology (T.M.), Nagoya University Graduate School of
Medicine, Nagoya; and the Department of Pathology and Tumor Biology (Y.-i.N.),
Kyoto University Graduate School of Medicine, Kyoto, Japan.
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Rat (Kidney Blood
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Mahmoud M. Khattab Adel Mostafa Othman Al-Shabanah
Department of Pharmacology, College of Pharmacy, King Saud
University, Riyadh , Saudi Arabia
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Tomoji Mashimo, Birger Voigt, Takashi Kuramoto, and Tadao Serikawa
Institute of Laboratory Animals, Graduate School of Medicine,
Kyoto University, Kyoto, Japan
An X-Ray Diffraction Study on
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Ryuji Toh,* Masakazu Shinohara,* Tomofumi Takaya,* Tomoya Yamashita,* Shigeru
Masuda,*
Seinosuke Kawashima,* Mitsuhiro Yokoyama,* and Naoto Yagiy
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Department of Internal Medicine, Kobe University Graduate School of
Medicine,Kobe 650-0017, Japan; and ySPring-8/JASRI, Sayo, Hyogo 679-5198,
Japan
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(ONO-1301) attenuates bleomycin-induced pulmonary fibrosis in
mice (Am J Physiol Lung Cell Mol Physiol 290: L59-L65, 2006.)
Shinsuke Murakami,1,2
Noritoshi Nagaya,1,3 Takefumi Itoh,2 Masaharu Kataoka,1
Takashi Iwase,1
Takeshi Horio,3 Yoshinori Miyahara,4 Yoshiki Sakai,5 Kenji Kangawa,6 and
Hiroshi Kimura2
1Department of Regenerative Medicine and Tissue Engineering,
National Cardiovascular Center Research
Institute, Osaka; 2Second Department of Internal Medicine, Nara Medical
University, Nara; 3Department of
Internal Medicine, National Cardiovascular Center, Osaka; 4Department of
Cardiac Physiology, National
Cardiovascular Center Research Institute, Osaka; 5Ono Pharmaceutical Co., Ltd.
Research Headquarters,
Osaka; and 6Department of Biochemistry, National Cardiovascular Center
Research Institute, Osaka, Japan
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Peptide Obtained from Hen Ovotransferrin (Journal of the Chinese Chemical
Society, 2006, 53, 495-501)
Nai-Yuan Leea, Juei-Tang Chengb, Toshiki Enomotoc, Ichiro Nakamurad,
a Livestock Research Institute, Tainan 712, Taiwan, R.O.C.
b Department Pharmacology, College of Medicine, National Cheng Kung University,
Tainan 70101, Taiwan, R.O.C.
c Department of Food Science, Ishikawa Prefectural University, Nonoichi,
Ishikawa 9218836, Japan
d Institute of Animal Experimentation, School of Medicine, Hokkaido University,
Sapporo 0608589, Japan
Animal used : SHR
Evaluation of Blood Pressure Measured by Tail-Cuff Methods (without
Heating) in Spontaneously Hypertensive Rats (Biol. Pharm. Bull. 29(8)
1756-1758 (2006) Vol. 29, No. 8
KUBOTA Yoko1
UMEGAKI Keizo2
KAGOTA Satomi 3
TANAKA Naoko 4
NAKAMURA Kazuki 5
KUNITOMO Masaru 6
SHINOZUKA Kazumasa 7
1 Department of Biopharmaceutics, Nihon Pharmaceutical
University
2 National Institute of Health and Nutrition
3 Department of Pharmacology, School of Pharmaceutical Sciences, Mukogawa
Women's University
4 Department of Pharmacology, School of Pharmaceutical Sciences, Kyushu
University of Health and Welfare
5 Department of Pharmacology, School of Pharmaceutical Sciences, Mukogawa
Women's University
6 Department of Pharmacology, School of Pharmaceutical Sciences, Mukogawa
Women's University
7 Department of Pharmacology, School of Pharmaceutical Sciences, Mukogawa
Women's University
Mechanical ventilation with 40% oxygen
reduces pulmonary expressionof genes that regulate lung development and
impairs alveolar septation in newborn mice
(Am J Physiol Lung Cell Mol Physiol 293:1099-1110, 2007. )
Richard D. Bland, Lucia M. Mokres, Robert Ertsey, Berit E. Jacobson, Shu Jiang,
Marlene Rabinovitch,
Liwen Xu, Eric S. Shinwell, Feijie Zhang, and Matthew A. Beasley
Department of Pediatrics, Stanford University School of
Medicine, Stanford, California
Mechanical ventilation uncouples synthesis
and assembly of elastin and increases apoptosis in lungs of
newborn mice.
(Am J Physiol Lung Cell Mol Physiol 294:L3-L14, 2008. )
Richard D. Bland, Robert Ertsey, Lucia M. Mokres, Liwen Xu, Berit E. Jacobson,
Shu Jiang,
Cristina M. Alvira, Marlene Rabinovitch, Eric S. Shinwell, and Anjali Dixit
Department of Pediatrics, Stanford University School of
Medicine, Stanford, California
Last Updated: June 06, 2008
